【摘要】 目的探讨肿瘤坏死因子-α(TNF-α)在热疗抑制肿瘤侵袭性过程中的作用。方法热处理大鼠恶性胶质瘤细胞(C6细胞)和胶质瘤大鼠后,放射免疫法监测培养液和脑胶质瘤组织内TNF-α的浓度;免疫组化法检测经热疗/TNF-α/生理盐水处理过的胶质瘤组织内增殖细胞核抗原(PCNA)蛋白的表达。利用Transwell构建肿瘤侵袭模型,通过结晶紫染色法检测肿瘤侵袭性。电镜观察C6恶性胶质瘤大鼠肿瘤血管内皮细胞的凋亡。结果热疗可增加C6细胞培养液和胶质瘤大鼠肿瘤组织内的TNF-α含量及降低胶质瘤侵袭性,均于热疗后120 min时达高峰(P<0.01)。热疗与TNF-α单独作用于胶质瘤大鼠后,均可引起胶质瘤大鼠肿瘤血管内皮细胞的凋亡,且TNF-α引起内皮细胞的凋亡水平与热处理后C6细胞培养液中TNF-α含量一致。结论热疗可能是通过增加TNF-α引起肿瘤血管内皮细胞凋亡而抑制了肿瘤侵袭性。更多还原

【Abstract】 Objective To explore role of tumor necrosis factor-α(TNF-α) during hyperthermia-induced tumor invasiveness decreases process.Methods Immunohistochemical methods were applied to detect proliferating cell nuclear antigen(PCNA) of glioma tissue treated by hyperthemia/TNF-α/normal saline(NS),radioimmunoassay was used to monitor TNF-α content of culture medium and brain glioma tissue after heat treated tumor-bearing rat and rat glioma cell line(C6 cell).Electron microscopy was performed to examine tumor vascular endothelial cell apoptosis.Results Hyperthemia not only increased the content of TNF-α of C6 rat glioma cell culture medium and tumor tissue,but also decreased glioma invasiveness.Hyperthemia and TNF-α can caused apoptosis of tumor vasculature endothelial cells.TNF-α caused tumor vascular endothelial cell apoptosis levels had the same trend with TNF-α content of C6 cell culture fluid after hyperthemia.Conclusion Hyperthemia induced tumor vascular endothelial cell apoptosis and inhibited tumor invasiveness through to increased TNF-α content in tumor tissue.更多还原