【摘要】 目的探讨肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)对大鼠心肌成纤维细胞(CFs)增殖和胶原合成的影响。方法用胰酶消化法培养新生Wistar大鼠CFs,加入重组人TWEAK(rhTWEAK)干预48 h,采用四甲基偶氮唑蓝(MTT)法测定细胞增殖,qRT-PCR检测Ⅰ型和Ⅲ型胶原mRNA,Western blot检测Ⅰ型胶原蛋白表达。结果 rhTWEAK作用后CFs的细胞数目明显增加,Ⅰ型和Ⅲ型胶原mRNA表达上调,同时Ⅰ型胶原蛋白表达显著增强。结论 TWEAK具有促进大鼠心肌成纤维细胞增殖和胶原合成的作用。更多还原

【Abstract】 Objective To investigate the effects of tumor necrosis factor-like weak inducer of apoptosis(TWEAK) on the proliferation and collagen synthesis of cultured rat cardiac fibroblasts(CFs).Methods Cultured CFs from neonatal Wistar rats were isolated by trypsinization,treated with recombinant human TWEAK(rhTWEAK),and examined after 48 h.The CFs proliferation was examined by MTT,and the expressions of collagen Ⅰ and Ⅲ mRNA and protein were determined by qRT-PCR.The expression of collagen Ⅰ protein was determined by Western blot.Resultsrh TWEAK significantly increased the number of CFs and elevated the expression levels of collagen Ⅰ and Ⅲ mRNA and collagen Ⅰ protein.Conclusion TWEAK can promote proliferation and collagen synthesis of rat CFs.更多还原