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纤维素酶对象草玉米秸秆混合青贮品质的影响

Effect of Cellulase on Quality of Napier Grass and Corn Straw Mixed Silage

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【作者】 陈鑫珠; 张文昌; 张建国; 李国栋; 廖惠珍; 陈炎芳; 郑东升; 庄益芬;

【Author】 CHEN Xin-zhu1,2,ZHANG Wen-chang1,ZHANG Jian-guo2,LI Guo-dong2,LIAO Hui-zhen3, CHEN Yan-fang1,ZHENG Dong-xu1,ZHUANG Yi-fen1﹡(1.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China; 2.College of Agriculture,South China Agricultural University,Guangzhou,510642,China; 3.Fuzhou Jin Bai Hui Biotechnology Co.,Ltd,Fuzhou 350012,China)

【机构】 福建农林大学动物科学学院; 华南农业大学农学院; 福州金佰慧生物技术有限公司;

【摘要】 为探明纤维素酶(cellulase,CEL)对象草(Pennisetum purpureum Schum)和甜玉米(Zea mays L.var.rugosa Bonaf.)秸秆混合青贮品质的影响。在象草:玉米秸秆为70:30(鲜样质量比)的混合原料中添加CEL进行青贮调制,添加水平分别为0%(对照)、0.001%(1U/g)、0.005%(5U/g)和0.025%(25U/g)。每个处理设3次重复。在常温条件下贮存60d,开封后测定青贮的pH、乳酸、乙酸、丙酸、丁酸,氨态氮等。结果表明,CEL能极显著减少青贮的中性洗涤纤维和半纤维素含量(P<0.01),极显著增加乳酸含量(P<0.01)。在4个处理组中,0.005%CEL组干物质和干物质回收率最高,并且该处理组的pH、乙酸、氨态氮和酸性洗涤纤维极显著(P<0.01)低于其他处理组,乳酸和可溶性碳水化合物极显著(P<0.01)高于其他处理组。上述结果表明,添加0.005%CEL的处理效果最佳。

【Abstract】 In order to study the influence of cellulase(CEL) additive on the quality of napier grass(Pennisetum purpureum) and corn straw(Zea mays l.var.Rugosa Bonaf.) mixed silage,the mixed ratio of napier grass to corn straw at 70:30 was treated with cellulase at the levels of 0.000%(control),0.001%(1 U/g),0.005%(5 U/g) and 0.025%(25 U/g).Ensilaged for 60 days at normal temperature,the fermentational indexes were measured with three replicates for each treatment.The results indicated that cellulose can significantly reduce neutral detergent fiber and hemi-cellulose(P<0.01) and increase lactic acid(P<0.01).The dry matter,dry matter recovery were the highest in silage with 0.005% cellulose among the four treatments,of which the pH,acetic acid,ammonia nitrogen and acid detergent fiber were significantly lower than other treatments while LA and WSC were higher(P<0.01).The results showed that adding 0.005% cellulase was the best for napier grass and corn straw mixed silage.

【关键词】 纤维素酶; 象草; 玉米秸秆; 青贮; 发酵品质;
【Key words】 cellulase; napier grass; corn straw; silage; quality;
【基金】 福建省教育厅科技项目(JK2009010)
  • 【文献出处】 家畜生态学报 ,Acta Ecologiae Animalis Domastici , 编辑部邮箱 ,2011年06期
  • 【分类号】S816.53
  • 【被引频次】21
  • 【下载频次】186
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