【摘要】 合成了聚(1-乙烯-3-乙基咪唑溴代盐)(poly(ViEtIm+Br-))聚合离子液体,并通过π-π堆积将其固载到多壁碳纳米管上,得到了聚离子液体-碳纳米管复合物.以15mg复合物的填充柱为固相吸附剂,在顺序注射系统中研究了其对白蛋白的在线固相萃取分离纯化.在pH5.2时,对2mL溶液中(50μgmL-1)白蛋白的吸附效率为91%.0.04molL-1柠檬酸盐缓冲溶液可定量洗脱吸附的白蛋白.整个分离纯化过程耗时500s,富集倍数为5.用该法对人全血中的白蛋白进行分离纯化,经SDS-PAGE凝胶电泳验证,得到了纯度较好的白蛋白.更多还原

【Abstract】 Polymerized ionic liquid(PIL),poly(1-vinyl-3-ethylimidazolium bromide)(poly(ViEtIm+Br-)),is synthesized and it is immobilized onto the surface of multi-walled carbon nanotubes(MWNTs) via π-π stacking,giving rise to a PIL-MWNTs composite.15 mg of the composite is packed into a mini-column for the on-line isolation/purification of bovine serum album(BSA) in a sequential injection system.A 91% adsorption of BSA in 2 mL solution(50 μg mL-1,pH 5.2) is achieved.The retained BSA can afterwards be quantitatively recovered with 0.04 mol L-1 citrate buffer.The entire isolation process takes 500 s giving rise to an enrichment factor of 5.The practical applicability of this on-line protocol is demonstrated by processing human whole blood for the isolation of human serum album,and satisfactory results are obtained by assay with SDS-PAGE.更多还原