【摘要】 丙型病毒性肝炎是由丙型肝炎病毒(hepatitis C virus,HCV)引起的一种传染性疾病.发展对HCV抗原具有高亲和力高特异性的识别分子和检测方法对丙肝进行早期诊断具有非常重要的意义.我们通过SELEX筛选得到了能特异性识别HCV核心蛋白(core蛋白)的DNA核酸适体,建立了可对HCV core蛋白进行高灵敏检测的核酸适体-酶联免疫新方法,并成功应用于丙肝病人血清中HCV core蛋白的检测,有望发展成为简便、灵敏、低成本的HCV早期诊断和血清筛查新方法.更多还原

【Abstract】 Hepatitis C,caused by the hepatitis C virus (HCV),is a serious infectious disease without vaccine.The development of methods and reagents for simple and sensitive HCV proteins detection is very important for the early stage diagnosis of HCV infection.In this work,we selected a DNA aptamer C7 which can specifically bind with HCV core antigen.Using this aptamer,we developed an aptamer-ELISA method to detect HCV core protein in both buffer and serum.The method was successfully used to diagnose HCV infected patients’ serum.It provided a new approach for easy,sensitive and low-cost blood screening and diagnosis of HCV infection.更多还原