【摘要】 目的观察血管紧张素转换酶(ACE)下调对胰腺癌细胞株SW1990运动侵袭能力的影响。方法设计并体外合成靶向ACE的小干扰RNA,将其转染到SW1990细胞中,蛋白质印迹法检测转染前后细胞中ACE蛋白的表达,采用Transwell小室检测转染前后细胞运动侵袭能力的改变。结果 ACE siRNA能有效降低SW1990细胞中ACE的表达。抑制SW1990细胞中ACE的表达后,SW1990细胞的运动能力明显下降,穿过人工基底膜的细胞数显著低于对照组(P<0.05)。结论核糖核酸干扰下调ACE表达能明显抑制胰腺癌细胞SW1990在体外的运动侵袭能力。更多还原

【Abstract】 Objective To investigate the effect of silenced ACE on migration and invasion on pancreatic cancer cell line SW1990.Methods ACE siRNA was designed and transfected into pancreatic cancer cell line SW1990.The expression of ACE protein in SW1990 was detected by Western blot.The capability of moving and invasion of cancer cells were tested by Transwell.Results SW1990 protein expression was suppressed after ACE siRNA transfection.The migrational and invasional ability of SW1990 cells transfected with ACE siRNA was significantly decreased compared with untransfected control cells(P<0.05).Conclusions Deletion of ACE gene can significantly inhibit the migration and invasion in SW1990 cells.更多还原