【摘要】 目的研究文昌鱼多糖(Polysaccharide fromBranchiostoma belcheri,PBB)对荷S180小鼠免疫功能的影响。方法 Horn法检测PBB在小鼠体内应用的安全性(LD50);建立S180荷瘤鼠模型,随机分组,实验组分别灌胃给予25、50、100mg.kg-1PBB,每日一次,持续2w。测定PBB对荷瘤鼠的抑瘤率、胸腺指数、脾指数的影响;全自动生化分析仪检测各组小鼠血浆免疫球蛋白IgA、IgG、IgM含量;放射免疫法检测各组小鼠血清IL-6和TNF-α水平。结果 PBB小鼠体内给予未见明显的中毒症状,无动物死亡,其LD50>3160mg.kg-1;给予上述PBB剂量2w后,抑瘤率分别达到39.1%、47.5%和53.4%;实验组IgA、IgG、和IgM含量、TNF-α和IL-6水平以及胸腺和脾指数均较模型组明显提高。结论文昌鱼多糖毒性低,使用安全,可明显抑制S180肉瘤的生长,其作用机制可能与PBB提高机体的免疫功能有关。更多还原

【Abstract】 Objective To investigate the effects of polysaccharides from Branchiostoma belcheri(PBB) on the immune functions in S180 tumor-bearing mice.Methods The toxicity(LD50) of PBB was estimated by Horn assay,the transplant tumor model of S180 mice was established and they were randomly divided into six groups:the model group,the normal group,the PBB group(25,50,100 mg·kg-1·d-1) and the cyclophosphamide group.The tumor inhibition rates and the indices of thymus and spleen were determined.The concentrations of IgA,IgG and IgM in plasma were detected by an automatic biochemistry analyzer.The levels of IL-6 and TNF-α in serum were determined by RIA.Results The PBB showed low toxicity with its LD50 more than 3160 mg·kg-1.With the doses of 25,50,100 mg·kg-1 PBB ig.for 2 w,the tumor inhibition rates were 39.1%,47.5% and 53.4%,respectively.The concentrations of IgA,IgG and IgM,and the levels of TNF-α and IL-6,as well as the indices of thymus and spleen were increased in PBB groups obviously.Conclusion PBB showed antitumor activity and some of the mechanisms may be explained by strengthening immunologic function in mice.更多还原