【摘要】 目的探讨昼夜节律基因hPer1和hPer2对食管癌细胞株(Eca-109)放射敏感性的影响。方法体外培养食管癌Eca-109细胞株,通过避光和乙酰肉豆蔻佛波酯(PMA)分别诱导昼夜节律基因hPer1和hPer2节律性的表达,用RT-PCR检测出表达高峰和低谷。在hPer1和hPer2表达的高峰和低谷时,分别用6mV-X射线给予照射,通过Tunel及流式细胞术,检测肿瘤细胞的凋亡。结果昼夜节律基因表达高峰时照射与低谷时照射比较,肿瘤细胞凋亡率减少(P<0.05)。结论昼夜节律基因hPer1和hPer2高表达,能够降低食管癌细胞的放射敏感性。更多还原

【Abstract】 Objective To investigate the effects of circadian rhythm genes-hPer1 and hPer2 on radiosensibility of esophageal cancer cell lines Eca-109.Methods The circadian rhythm expression of hPer1 of esophageal cancer cell lines,Eca-109,was induced with dark-culture.The circadian rhythm expression of hPer2 of Eca-109 was induced with phorbol 12-myristate 13-acetate(PMA).The expression of hPer1 and hPer2 was detected with PT-PCR.Eca-109 cells were irradiated at peak(PG-hPer1,PG-hPer2) and nadir(NG-hPer1,NG-hPer2) of hPer1 and hPer2 with 6mV-X-ray respectively.The apoptosis rate of Eca-109 were evaluated with TUNEL and flow cytometry.Results The apoptosis rate of Eca-109 cells of PG-hPer1 and PG-hPer2 were lower than those of NG-hPer1 and NG-hPer2(P<0.05).Conclusion hPer1 and hPer2 over-expression can decrease the radiosensibility of esophageal cancer cell lines Eca-109.更多还原