【摘要】 以海洋赤潮藻为材料,从宁波市象山港附近海域采取水样,经反冲超滤法对水样进行浓缩处理后,用其对培养的赤潮藻细胞进行感染,分离到了一株能裂解牟勒氏角毛藻的病毒,经反复的液体感染,建立了稳定的藻病毒-宿主藻系统,命名为牟勒氏角毛藻病毒。该病毒具有很强的感染力,稀释108倍以后仍然具有明显的感染效果。病毒纯化后,电镜观察显示此株病毒为球形多面体结构,直径约为58nm;电镜切片观察显示,感染后的藻细胞中充满了大量的游离的和组装好尚未释放的病毒粒子。经宿主范围鉴定,此株病毒具有严格的宿主专一性。通过分子生物学初步鉴定,其基因组大小约为23kb。此株病毒尚未有报道,是一种新发现的海洋藻类病毒,具有进一步研究的价值。更多还原

【Abstract】 Water from Xiangshan harbor and the East China Sea were collected and concentrated by backflushing ultrafiltration, and then were used as infecting medium for growing marine redtide algae as host. A novel virus named Chaetoceros muelleri virus was found that lysed C. muelleri. The virus was isolated by repeated liquid infection and a stable Phycoviruses-Host system of algae was established. Infection via Most-Probable-Number analysis revealed that the virus had a strong infectious power;it was found significantly infectious even after 108-fold dilution. The virus was purified through saccharose gradient ultracentrifugation. Phosphotungstic acid negative staining analysis revealed the viral capsid of C. muelleri virus had a distinct icosahedral shape with a diameter of 58nm. Electron microscopic section revealed that there were many free particles and some assembled viral particles ready to be released in the infected algae cells. Identification of the host range confirmed that the virus had a strict host specificity for C. muelleri cells but not others. This novel C. muelleri virus genome is about 23kb through molecular characterization. No known report for this virus, it is a novel marine phycovirus worthy to be studied.更多还原