【摘要】 本文研究了不同的Cd2+浓度与磷酸盐浓度交互作用对小球藻(Chlorella sp.)和微绿球藻(Nannochloris oculata)生长及叶绿素荧光特性的影响。三因子方差分析结果表明,Cd2+浓度、磷酸盐浓度、胁迫时间及三者的交互作用对小球藻和微绿球藻的叶绿素荧光特性、叶绿素相对含量以及细胞密度均具有显著影响(P<0.05)。相关性分析结果表明,2株绿藻的主要荧光参数Fv/Fm(PSⅡ的最大光化学量子产量)、Fv/Fo(PSⅡ的潜在活性)、Yield(PSⅡ的实际光能转化效率,=ΦPSⅡ)以及相对电子传递效率(rETR)均与Cd2+浓度呈显著的负相关关系。在低磷酸盐处理组(2.27μmol/L),小球藻和微绿球藻的细胞密度、主要荧光参数(Fv/Fm、Fv/Fo、Yield、rETR)以及叶绿素相对含量均明显低于对照组和高磷酸盐处理组(290.4μmol/L),表现为明显的磷限制现象。在高磷酸盐处理组,上述参数均明显高于低磷酸盐处理组和对照组,说明富磷条件下可以一定程度地减轻重金属Cd2+对小球藻和微绿球藻的毒害作用。更多还原

【Abstract】 The interaction effects of different Cd2+ and P concentrations on the growth and chlorophyll fluorescence parameters(Fv/Fm,Fv/Fo,Yield and rETR) of Chlorella sp.and Nannochloris oculata(obtained from the Microalgae Culture Center(MACC),Ocean university of China) were investigated.The three-factor analysis of variance results indicated that Cd2+ concentrations,P concentrations,stress time and their interaction all had significant effects on the chlorophyll fluorescence parameters,relative chlorophyll content and cell density of Chlorella sp.and N.oculata(P<0.05).The correlation results showed that the main fluorescence parameters(Fv/Fm,Fv/Fo,Yield and rETR) were negative correlations with Cd2+ concentrations in Chlorella sp.and N.oculata,both decreased with increasing Cd2+ concentrations.Further more,compared to the control and the high P concentration treatment,the parameters Fv/Fm,Fv/Fo,Yield and rETR,relative chlorophyll content and cell density of the 2 microalgal strains remarkably decreased in low P concentration(2.27μmol/L),which showed obvious phosphorus limitation.Otherwise,the above parameters reached their highest values at high P concentration(290.4μmol/L),which showed high P concentration could reduce toxicity under Cd2+ stress.更多还原