【摘要】 目的观察正丁胺基罗沙替丁乙酸酯(BRA)对大鼠实验性胃溃疡边缘黏膜细胞凋亡及凋亡相关基因Bcl-2蛋白表达的影响,探讨其治疗胃溃疡的作用机制。方法制备大鼠乙酸烧灼型胃溃疡模型,分别给予不同剂量BRA治疗15 d后,观察溃疡边缘黏膜细胞凋亡、凋亡相关基因Bcl-2的表达情况。结果 BRA能显著抑制胃溃疡边缘黏膜细胞凋亡,同时促进凋亡抑制基因Bcl-2的表达,与模型组相比较:14、28 mg·kg-1剂量组的效果更为显著。结论 BRA治疗胃溃疡的作用机制可能与抑制凋亡及增加Bcl-2蛋白表达有关。更多还原

【Abstract】 OBJECTIVE To observe the effect of Butylamine roxatidine acetate(BRA)on the apoptosis and apoptosis-related gene Bcl-2 protein expression of the mucosal cells in the ulcer border of rats and to investigate the mechanism of the effect of BRA on rat gastric ulcer.METHODS The rat gastric ulcer model was prepared by acetic acid burn and was treated for 15 d with different doses of BRA to observe the apoptosis of ulcer edge mucosal cell and Bcl-2 expression of apoptosis related genes.RESULTS BRA significantly inhibited gastric mucosal cell apoptosis compared with that of the model group and apoptosis gene Bcl-2 expression was increased significantly at the dose of 14,28 mg BRA/kg rat.CONCLUSION Anti-ulcer mechanism of BRA may be related to inhibition of apoptosis and increased Bcl-2 protein expression.更多还原