【摘要】 目的分析测定芜菁子中油脂的组成及含量。方法采用索氏提取法提取芜菁子中的油脂,甲酯化后,GC-MS法分析其油脂中脂肪酸的成分;将芜菁子油脂皂化,以2,4-二溴苯乙酮为衍生化试剂,采用RP-HPLC法检测,流动相为乙腈-水(80∶20),流速1 mL.min-1,各脂肪酸在40 min内可很好地分离。结果芜菁子中含有油酸、亚油酸、亚麻酸、硬脂酸、软脂酸、芥酸等6种脂肪酸,含量分别为12.5%、19.6%、8.9%、7.4%、15.8%、28.2%。结论所建方法操作简便,灵敏度高,准确可靠,可用于芜菁子油中脂肪酸的含量测定。更多还原

【Abstract】 OBJECTIVE To determine compositions and contents of the oil in Brassica rapa L.seed.METHODS The oil in Brassica rapa L.seed was extracted by Soxhlet extraction.Oil components were analyzed by GC-MS.After saponified,the fatty acids were derivated with 2,4,-dibromo-acetophenone and detected at 254 nm by RP-HPLC.Acetonitrile-water(80:20)was used as a mobile phase and flow rate was 1 mL·min-1.The fatty acid were separated and determined within 40 min.RESULTS Six kinds of fatty acid were found in Brassica rapa L.seed.They were oleic acid(12.5%),linoleic acid(19.6%),linolenic acid(8.9%),stearic acid(7.4%),palmitic acid(5.8%),erucic acid(18.2%).CONCLUSION The method is simple,sensitive,accurate and reliable.It can be used to determine the contens of fatty acids in Brassica rapa L.seed.更多还原