【摘要】 本文研究了不同因素对脱氧核糖核酸(DNA)毛细管电泳迁移行为的影响。采用毛细管无胶筛分电泳法,对不同片段长度DNA进行分离检测,考察DNA片度长度、电场强度、聚合物浓度及分子量等因素对DNA迁移行为的影响。实验结果显示,DNA迁移时间随其长度的增加而延长;电场强度越高,DNA迁移时间越短,分离效果变差;DNA在高浓度聚合物介质中的迁移时间较低浓度中延长;聚合物分子量越高,DNA迁移时间越长。基于对大片段DNA的分离效果考虑,毛细管无胶筛分实验中应限制高场强的使用;为了实现较小DNA片段的满意分离,应使用分子量较小的筛分介质,反之亦然。更多还原

【Abstract】 Effects of various factors on deoxyribonucleic acid(DNA)separation by capillary non-gel sieving electrophoresis were investigated in this paper.DNA was separated by capillary non-gel sieving electrophoresis.Effects of DNA fragment length,the electric field strength,polymer concentration,and polymer molecular weight on the migration of DNA were all discussed.Experimental results show that DNA migration time increased with its length extending.DNA migration time decreased with the increasing of electric field strength,and the separation efficiency become worse.DNA spends longer time in capillary electrophoresis when separated in polymer sieving media with higher concentration or molecular might.According to separation efficiency of longer DNA,the higher electric field strength was not recommended in capillary non-gel sieving electrophoresis.In order to obtain satisfied separation of shorter DNA fragments,we should use polymer with small molecular weight as sieving material,and vice versa.更多还原