【摘要】 在pH9.00的tris-HCl缓冲溶液中,甲酚红与芬布芬反应形成离子缔合物,使甲酚红溶液褪色。实验结果表明:最大褪色波长位于569.0nm,芬布芬浓度在0.05086～10.17μg/mL范围内遵循比尔定律,回归方程为ΔA=0.1702c+0.0107(c=μg/mL),相关系数为r=0.9996,表观摩尔吸光系数为4.537×104L.mol-1.cm-1。据此建立了测定药物制剂和生物样品中芬布芬含量的褪色分光光度法,样品测定平均回收率为97.97%～101.6%。更多还原

【Abstract】 In pH9.00 tris-HCl buffer medium,Cresol Red reacted with Fenbufen to form an ion association complex,which caused the fading of the Cresol Red solution.The results showed that the maximum fading wavelength is located at 569.0nm.The concentration of FBF obeyed the Beer′s law in the range of 0.05086～10.17μg/mL and the regression equation wasΔA=0.1702c+0.0107(c=μg/mL)with the correlation coefficient was 0.9996,the apparent molar absorptivity was 4.537×104 L·mol-1·cm-1.Fading spectrophotometric method has been developed for the determination of medicinal preparation and biological samples with Fenbufen.The recovery of sample was within the the range of 97.97～101.6%.更多还原