【摘要】 以十六烷基胺与十六烷基溴为原料,通过取代反应与缩合反应等5步反应分别合成人工脂质(肽脂质)与人工受体。利用自组装技术在脂质体上构建了超分子开关体系,其中磷酸吡哆醛(PLP)为信号分子,铜离子做为中间信使。在没有调控信号时,铜离子与乳酸脱氢酶结合,抑制酶的活性;当加入信号分子PLP时,信号分子与人工受体作用形成席夫碱,席夫碱与乳酸脱氢酶竞争铜离子,解除铜离子对乳酸脱氢酶活性的抑制,恢复乳酸脱氢酶的催化活性。更多还原

【Abstract】 A cationic peptide lipid named N,N-dihexadecyl-Nα-[6-(trimethylammonio)hexanoyl]-alaninamide bromide(N+C5Ala2C16)and a artificial receptor were synthesized with 1-hexadecylamine and 1-bromohexadecane through five steps,including substitution and condensation reactions.A self assembled superamolecular switch system was obtained based on liposome.In this system,pyridoxal 5′-phosphate acts as artificial signal,Cu2+ acts as a mediator.Without signal molecules,Cu2+ bound LDH and inhibited LDH activities.When signal molecule——PLP was added to this system,PLP attached to liposome surface,and reacted with the amino group of the artificial receptor,and formed Schiff base,and captured copper cation,and led to the recovery of LDH activities which has been inhibited by copper cation.更多还原