【摘要】 目的通过观察少突胶质细胞系基因-髓鞘碱性蛋白(Golli-MBP)在口腔扁平苔藓(OLP)患者外周血单个核细胞中的表达,探索OLP的发病机制。方法采用密度梯度离心法分离20例充血糜烂型、16例光滑型OLP患者,以及19例对照组的外周血单个核细胞,分别用逆转录-聚合酶链反应(RT-PCR)法和Western blot法检测3组研究对象外周血单个核细胞中Golli-MBP mRNA和蛋白的表达。结果充血糜烂型和光滑型OLP患者中Golli-MBP mRNA和蛋白的表达水平均高于对照组,且差异有统计学意义(P<0.01);但充血糜烂型和光滑型OLP患者组间表达的差异均无统计学意义(P>0.05)。结论 Golli-MBP在OLP的发病过程中起着重要作用。更多还原

【Abstract】 Objective The aim of this study was to determine the expression of genes of the oligodendrocyte lineage-myelin basic protein(Golli-MBP) in peripheral blood mononuclear cell(PBMC) in oral lichen planus(OLP) and to further understand the pathogenesis of OLP.Methods PBMC was obtained by density gradient centrifugation,and the expres-sion of Golli-MBP in PBMC was investigated in erythematous/erosive OLP(20 cases),reticular OLP(16 cases) and normal controls(19 cases) using reverse transcription-polymerase chain reaction(RT-PCR) and Western blot methods.Results RT-PCR results showed that Golli-MBP mRNA was overexpressed in erythematous/erosive and reticular OLP as compared with normal control group(P<0.01).Western blot assay indicated that erythematous/erosive and reticular OLP patients had a higher expression level of Golli-MBP protein in PBMC than normal controls(P<0.01).However, there were no significant differences between erythematous/erosive and reticular groups in Golli-MBP mRNA and protein expression(P>0.05).Conclusion The data accumulated here strongly indicate that Golli-MBP was involved in the pa-thogenesis of OLP.更多还原