【摘要】 目的观察纯钛试件应用等离子镀膜法镀制纳米渗氮类金刚石(N-DLC)薄膜后的细菌黏附变化,以期对临床应用有所指导。方法采用等离子镀膜机,在纯钛试件表面沉积N-DLC薄膜、TiN薄膜、阳极氧化膜以及空白对照,然后将其黏附于树脂基托表面,实际使用6个月后,扫描电子显微镜观察白色假丝酵母菌黏附试件情况;粗糙度检测仪对比检测试件粗糙度变化。结果白色假丝酵母菌在N-DLC膜表面附着数量比其他组试件明显少(P<0.05),且菌体生长不良,处于抑制状态;阳极氧化膜和空白对照试件表面黏附菌量最多,且菌体生长旺盛。N-DLC膜表面粗糙度在戴用前后变化最小(P<0.05)。结论纯钛表面镀制N-DLC膜在口腔实际应用过程中可以明显降低白色假丝酵母菌的黏附量,从而降低罹患义齿性口炎的风险。更多还原

【Abstract】 Objective The aim of this study was to observe the change of bacterial adhesion on pure titanium coa-ted with nitrogen-diamond like carbon(N-DLC) films and to guide the clinical application.Methods N-DLC was deposited on titanium using ion plating machine,TiN film,anodic oxide film and non-deposition were used as control,then made specimens adhering on the surface of resin denture base for 6 months.The adhesion of Saccharomyces albicans on the titanium surface was observed using scanning electron microscope,and the roughness was tested by roughness detector.Results The number of Saccharomyces albicans adhering on diamond-like carbon film was signi-ficantly less than on the other groups(P<0.05),and the growth of bacterial cell was inhibited and in a poor state.The largest number of adhesion and cell strains grew well on anodic oxide film group and non-deposition control group.The change of surface roughness of N-DLC film was less than other group(P<0.05).Conclusion Pure titanium coated with N-DLC film reduced the adhesion of Saccharomyces albicans after clinical application,thereby reduced the risk of denture stomatitis.更多还原