【摘要】 目的研究钛片表面粗糙度和氧化膜对成骨细胞黏附的影响,为选择临床种植体的表面处理方法提供依据。方法 250片纯钛钛片分为5组。采用直径分别为108～130μm(S1组)、216～301μm(S2组)、356～411μm(S3组)TiO2砂对钛片表面进行喷砂处理;另外一组钛片采用钛浆喷涂(TPS)技术处理,设为TPS组;最后一组以600目SiC砂纸打磨组作对照(S0组)。分别测定钛片表面的粗糙度和氧化膜结构。在5组钛片表面进行成骨细胞培养,比较各组钛片表面成骨细胞在黏附1、4、12及24 h时的形态及黏附率。结果 S3组钛片表面氧化膜结构完整、连续,其表面的成骨细胞形态优于其他组。黏附1、4 h时,S3组黏附率高于其他各组(P<0.05);黏附12、24 h时,S3和TPS组黏附率高于其他各组(P<0.05);黏附4、12、24 h时,S1、S2、S3和TPS组黏附率均高于S0组(P<0.05)。结论表面粗糙度较高的喷砂表面的粗糙度和氧化膜结构更有利于成骨细胞黏附。更多还原

【Abstract】 Objective To study the effects of surface roughness and titanium dioxide(TiO2) layers on commercially pure titanium(cp-Ti) substrates on attachment of osteoblasts in vitro.Methods 250 pure titanium slices were divided into five groups.Osteoblasts were cultured on five cp-Ti substrates of ground,which blasted with 108-130 μm(S1),216-301 μm(S2),356-411 μm(S3) TiO2 particles and titanium-sprayed plasma(TPS) surfaces,surfaces prepared by hand grinding with SiC paper to 600 grits served as control(S0).Surface average roughness and the TiO2 film structure was evaluated.For morphology and attachment measurement,osteoblasts were cultured for 1,4,12 and 24 h,evaluated by scanning electronic microscope(SEM) observation and MTT assay.Results Osteoblasts spread well on the titanium surfaces.Further more,osteoblasts spread more well on S3 surfaces.After 1 and 4 h culture,the number of cells on S3 surfaces was the highest(P<0.05).The number of cells on S3 surfaces was the same(P>0.05) as TPS surfaces and higher than other groups(P<0.05) after 12 and 24 h.The number of cells of all experimental groups were higher than S0 surfaces after 4,12 and 24 h(P<0.05).Conclusion It was concluded that the coarse TiO2 particles blasted surface would optimize initial osteoblast responses.更多还原