【摘要】 蛋白质的磷酸化过程是人体内翻译后修饰的重要一步,非正常的磷酸化会导致人体产生各种疾病。近年来,通过分析化学的手段研究磷酸化的过程取得了很大的进展。该文就蛋白激酶分析、磷酸化多肽的富集方面的国内外的研究进展进行了综述。更多还原

【Abstract】 The phosphorylation of proteins is one of the most important steps of posttranslational modifications. Many diseases are induced by aberrant phosphorylation. In the past decade, numerous analytical methods have been developed to profile the protein phosphorylation. In this review, we focus on the recent promising advances in the field of phosphorylated protein analysis and detailedly review new and established methods for probing protein kinase activity and the enrichment of phosphorylated peptide.更多还原