【摘要】 目的:同时对苹果中上述三种主要糖进行定量分析。方法:采用核磁共振波谱分析技术,确定了苹果中葡萄糖、果糖和蔗糖在重水中的各种构型,归属了苹果中葡萄糖、果糖和蔗糖的质子核磁共振谱的主要特征峰,以邻苯二甲酸氢钾作内标。结果:葡萄糖、果糖和蔗糖测定结果的相对标准偏差分别为4.2%、4.4%、5.1%,回收率分别为97.4%、96.6%、96.5%。结论:该法取样量少,直接定量,所得结果重现性好、准确度高,为苹果的品质鉴定提供一种简单可行的方法。更多还原

【Abstract】 Objective To determine the content of glucose,fructose and sucrose at the same time.Methods Various configurations of glucose,fructose and sucrose in the raw juice of apple were determined by NMR when dissolved in heavy water.The main characteristic peaks of glucose,fructose and sucrose in the raw juice of apple were attributed by 1H-NMR spectra,Potussium biphthalate was selected as an internal standard.Results RSD of determination results were 4.2%,4.4% and 5.1% to glucose,fructose and sucrose respectively,and recovery of them were 97.4%,96.6% and 96.5% respectively.Conclusion This method used only a little sample and could determine the contents of glucose,fructose and sucrose directly,the results were stable and repeatable,the precision and accuracy were quite well.This method was simple and available in the quality identification of apple.更多还原