【摘要】 目的:探讨曲古抑菌素A(trichostatin A,TSA)对耐伊马替尼细胞株K562R的增殖和凋亡的影响。方法:采用3-(4,5-二甲基-2-基)-5(3-羧基甲基苯基)-2-(4-磺酸苯基)-2H-四氮唑细胞毒试验法观察细胞增殖抑制,细胞凋亡率采用流式细胞仪凋亡试剂盒检测。结果:TSA单用能抑制K562R细胞的增殖,呈时间剂量依赖性,并能促进凋亡。与伊马替尼联用有明显协同作用。结论:TSA与伊马替尼联用具有明显抑制耐伊马替尼的K562R增殖和诱导凋亡的作用,可能为临床伊马替尼耐药的患者提供新的治疗药物。更多还原

【Abstract】 Objective To explore the effect of trichostatin A (TSA) alone and combination with imatinib on the proliferation and apoptosis of K562R cells.Methods 3-(4,5-dimethylthiazol-2-yl) -5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay was used to observe the proliferation of K562R cells and apoptosis was analyzed by annexin-Ⅴ/propidium iodide(PI) staining.Results After exposure to TSA,the proliferation of K562R cells was inhibited,and the effect was in both time- and dose-dependent manner.The apoptosis was induced.Combined with imatinib,the effect of proliferation inhibition was better.Conclusion TSA combined with imatinib can inhibit the proliferation of K562R cells and induce its apopotosis.TSA may become a new drug for imatinib-resistant patients.更多还原