Objective To analyze the distribution and subcellular localization of NOR1 alternative splice isoforms in human tissues and cell lines.Methods NOR1 open reading frame(ORF)was amplified from human fetal brain cDNA library and subcloned into pCMV/myc vector.The level of NOR1 mRNA in human tissues was determined by real-time RT-PCR.Region spanning exon 2 was amplified from cDNA or genomic DNA by specific primers and sequenced.The expression plas-mids of NOR1 were transfected into cells and immunofluoresence as...
【基金】
supported by the National Natural Science Foundation of China(81000883,30971147,30871282,30871365);
the Natural Science Foundation of Hunan Province(10JJ7003);
the Free Exploration Program of Central South University(201012200017);
the Graduate Degree Thesis Innovation Foundation of Central South University(2010ssxt048),P.R.China
【更新日期】
2011-09-02
【分类号】
R739.63
【正文快照】
Alternative splicing is a common phenomenon ineukaryotes[1].Alternative splicing has a significantand underappreciated role in expanding proteome di-versity.Studies have consistently reported a high rateof alternative splicing in the human genome,with35%-
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