【摘要】 以观赏凤梨‘名宝剑’的短缩茎为外植体,研究了不同激素及浓度组合对愈伤组织诱导和植株分化的影响,并通过形态学和组织细胞学观察了不同类型愈伤组织的超微结构和胚性愈伤组织的分化过程。结果表明:NAA对愈伤组织诱导的影响达到极显著水平(P=0.0019),其中0.5mg/L NAA最有利于提高愈伤组织的诱导率和胚性愈伤组织的比例,最佳诱导培养基为MS+2.0mg/L 6-BA+0.5mg/LNAA+2.0mg/L 2,4-D,诱导率高达80%;6-BA和NAA组合有利于加快胚性愈伤组织的分化和提高不定芽分化频率,胚性愈伤组织在MS+0.5mg/L 6-BA+0.1mg/L NAA培养基上分化效率达到100%。电镜观察发现:胚性愈伤组织结构紧密,细胞较小,细胞内含物丰富,细胞代谢旺盛;非胚性愈伤组织结构疏松,细胞体积巨大,内含大液泡,基本无内含物;胚性愈伤组织在分化过程中细胞形态存在很大差异。更多还原

【Abstract】 A system for embryogenic callus(EC) induction and plant regeneration of ornamental pineapple(Vriesea’Tiffany’) using stem as explant was developed.Callus was induced on MS medium supplemented with various concentrations of 6-BA,NAA and 2.0mg/L 2,4-D.Plantlet differentiation was induced on MS medium supplemented with NAA and 6-BA.The results showed that the best treatment in terms of response quality and mean number of EC for explants was 0.5mg/L NAA with 2.0mg/L 6-BA.The best medium for EC induction was MS+2.0mg/L 6-BA+0.5mg/L NAA +2.0mg/L 2,4-D with the induction frequency 80%.The best shooting regeneration of EC was obtained on MS supplemented with 0.1mg/L NAA and 0.5mg/L 6-BA with the maximum reaching 100%.Morphological and ultrastructure differences between EC and Non-embryogenic callus(NEC) were observed by electron microscopy during proliferation and differentiation phases.Structural cell organelles are abundant in the EC cells,neither NEC cells.The characteristics of the callus were described in the paper.更多还原