【摘要】 利用甲基化敏感扩增多态性(methylation-sensitive amplification polymorphism,MSAP)技术,研究不同聚乙二醇(PEG-6000)处理浓度下霍山石斛基因组DNA的甲基化水平和变化模式。结果表明:经5%、10%和15%PEG-6000处理的霍山石斛DNA甲基化比率分别为34.7%、32.9%和30.4%,均低于对照的37.2%,且甲基化水平与PEG-6000处理浓度呈显著负相关(r=-0.998)。与对照相比,5%、10%和15%PEG-6000胁迫下霍山石斛基因组DNA发生甲基化变化的位点比例为12.02%、12.50%和15.53%,去甲基化位点比例为8.39%、8.55%和8.45%;发生DNA的甲基化和去甲基化位点之比、DNA甲基化多态性均随着干旱胁迫的增强而逐步提高。更多还原

【Abstract】 The methylation-sensitive amplified polymorphism(MSAP) techniques were used to assess genetic and epigenetic variations of Dendrobium huoshanense under drought stress.Results showed that the level of global DNA methylation decreased with increasing of PEG-6000 concentrations.When the tube seedlings were treated with 5%,10%,15% of PEG-6000,the levels of DNA methylation dropped to 34.7%,32.9% and 30.4%,respectively,compared with that for the control group(37.2%).There was a significantly negative correlation(r =-0.998) between PEG-6000 and DNA methylation.In contrast with the control,under stress of 5%,10% and 15% PEG-6000,methylation and demethylation of DNA were 12.02%,12.50%,15.53% and 8.39%,8.55%,8.45%,respectively.The ratios of DNA methylation and demethylation,the polymorphism of DNA methylation increased gradually,accompanied with the enhancement of drought stress.更多还原