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转基因青蒿与其野生型的生长和抗逆性比较

COMPARISON OF THE GROWTH AND STRESS TOLERANCE BETWEEN TRANSGENIC Artemisia annua VARIETIES AND ITS WILD-TYPE

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【作者】 刘华蒋玲曦王金斌谭芙蓉吴潇朱宏赵凯唐克轩唐雪明

【Author】 LIU Hua1,2,3 JIANG Ling-xi1,3,4 WANG Jin-bin1,3 TAN Fu-rong1,3 WU-Xiao1,4 ZHU-Hong1,4 ZHAO Kai1,3 TANG Ke-xuan5 TANG Xue-ming1,3,4(1.Supervision,Inspection and Test Center for Environmental Safety of Genetically modified Crops of Ministry of Agriculture,Shanghai 201106;2.the College of Fisheries and Life Science of Shanghai Ocean University,Shanghai Ocean University,Shanghai 201306;3.Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106;4.Key Laboratory of Agricultural Genetics and Breeding,Shanghai Academy of Agricultural Sciences,Shanghai 201106;5.Plant Biotechnology Research Institute,Institute of Agricultural Biotechnology in Shanghai Jiao Tong University,Shanghai 200240)

【机构】 农业部转基因植物环境安全监督检验测试中心上海海洋大学水产与生命学院上海市农业科学院生物技术研究所上海市农业遗传育种重点实验室上海交通大学农业生物学院植物生物技术研究中心

【摘要】 在环境释放条件下,比较了转基因青蒿及其野生型受体的基本农艺性状、抗逆性及耐常用除草剂等特性。在旱、盐胁迫下,通过测定3种转基因品系和其野生型受体的叶片相对含水量、过氧化物酶及脯氨酸等生理指标,比较了转基因青蒿和野生型的耐受性。结果显示,转基因青蒿品系ANF176、GFH13的株高、茎粗显著低于其野生型受体,转基因青蒿品系SQS159的冠幅和千粒重显著高于其野生型受体。其他指标的比较试验研究显示转基因青蒿品系与其野生型受体没有显著差异性。在本试验条件下,这3种转基因青蒿品系具有与野生型类似的抗逆能力(耐盐性、耐旱性),转基因青蒿与野生型对2种除草剂克无踪和金都尔不具有耐受性。

【Abstract】 The basic agronomic characteristics of transgenic A.annua lines and the wild-type were evaluated,and the physiological responses to the drought and salt stress of three transgenic A.annua varieties and wild-type were compared by determining the relative water content of leaves,POD,proline and so on.The results indicated that there were significant differences in plant height and stem diameter between transgenic A.annua varieties ANF176,GFH13 and wild-type,in crown width and 1000-seeds weight between transgenic A.annua variety SQS159 and wild-type.The difference between three transgenic A.annua varieties and wild-type in other agronomic characteristics was not significant.Moreover,under the experiment condition transgenic A.annua varieties and the wild-type had similar stress tolerances to salt and drought.Three transgenic A.annua lines and the wild-type were not tolerant to the two herbicides,paraquat and s-metolachlor.

【基金】 上海市科技兴农重点攻关项目(沪农科攻字(2009)第6-4-2号);上海市科委科技攻关项目(08391911800);上海市科委创新平台建设项目(10DZ2294103)
  • 【文献出处】 核农学报 ,Journal of Nuclear Agricultural Sciences , 编辑部邮箱 ,2011年02期
  • 【分类号】S567.219
  • 【被引频次】4
  • 【下载频次】229
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