【摘要】 为了探讨远志皂苷对小鼠学习能力及海马CA3区突触形态的影响,把30只小鼠随机分为2组:药物组灌胃生药量为4.50 g/kg的远志皂苷溶液,对照组灌胃相等剂量的生理盐水,14 d后进行行为学训练,期间继续给药10 d.训练结束后,各取7只小鼠检测海马CA3区突触密度、活性带长度、突触间隙宽度、PSD厚度及穿孔突触的比例.结果发现:远志皂苷能明显提高小鼠的正确反应率,降低达标所需训练次数;使小鼠海马CA3区穿孔突触比例明显提高,突触密度增加,活性带增长,突触间隙变窄,PSD增厚.远志皂苷能明显提高小鼠的行为习得能力,其相关机制可能与提高突触形态的可塑性密切相关.更多还原

【Abstract】 To study the effects of polygala tenuigenin(TEN) on learning and synaptic configuration of hippocampal CA3 area in mice,30 mice were divided randomly into the control group and TEN group.All mice were orally given TEN in dose of 4.50 g/kg or saline for 14 days,and then were trained.The mice were given drug or saline during training period.After 10 days training,seven mice in each group were measured for the synaptic density(Nv),the length of active zone,the width of synaptic cleft,the thickness of postsynaptic density(PSD) and the ratio of perforated synapses in hippocampal CA3.The results were:(1) TEN could improve the rates of correct response and decrease the numbers of trails to reach the learning criterion.(2) TEN could increase the synaptic density,the ratio of perforated synapses,the length of active zone and the PSD thickness,and reduce the width of synaptic clef.These results showed that TEN markedly improved the behavior-learning in mice,which may be related to improvememt of the plasticity of synaptic morphology.更多还原