【摘要】 以怀地黄花药为材料,研究了激素、温度、品种及光照对怀地黄愈伤组织诱导的影响。结果表明:2,4-D、6-BA、KT和品种对怀地黄愈伤组织诱导均有一定影响,各因素的组合以2,4-D 2.0 mg/L+6-BA0.5 mg/L+KT 2.0 mg/L+串地龙为最佳,诱导率达到47%;4℃低温预处理3 d,诱导效果最佳,诱导率达到43%;黑暗条件下更有利于愈伤组织的诱导,并可以减少后期生长中的褐化现象。更多还原

【Abstract】 Using the anther of Rehmannia glutinosa Libosch as materials,several factors,including different hormone treatments,temperature,cultivars,and light on anther callus induction of Rehmannia glutinosa Libosch were studied.The result showed that 2,4-D,6-BA,KT,and cultivars had important effects on anther induction.2,4-D 2.0mg/L+6-BA 0.5mg/L + KT 2.0mg/L + Chuandilong was the best combination for callus induction.The pre-treatment at 4℃ for 3 days were the most conductive to callus induction with induction rate of 43%;It was more favorable to callus induction in darkness than in light,which reduced callus browning during the later growth.更多还原