【摘要】 通过对鸡白介素18(IL-18)原核表达工程菌BL21-ChIL-18遗传稳定性、表达产物的West-ern-blot及其经Sephadex G-100纯化后的ELISA和淋巴细胞转化试验分析,以检测其遗传稳定性及其表达产物的生物学活性。结果表明,该重组工程菌能稳定地表达鸡IL-18,表达产物不仅能够与抗鸡IL-18的多克隆抗体发生反应,而且具有刺激淋巴细胞增殖并诱导其分泌γ干扰素的作用,为研究鸡IL-18在鸡体内的生物学功能奠定了基础。更多还原

【Abstract】 In order to detect the heredity stability and bioactivity of prokaryotic engineering bac-teria strain BL21-ChIL-18,its heredity stability and Western-blot analysis of its products were performed and other bioactivity of Sephadex G-100 column purified products were analyzed by ELISA and MTT.The results showed that the recombinant engineering strains can stably express chicken IL-18.Expression products can react with polyclonal antibody anti-chicken IL-18 and induce T lymphocytes proliferation and secreting IFN-γ in vitro.This laid a foundation for further research and the biological function within the chicken body studies.更多还原