【摘要】 [目的]为探究钙离子、茉莉酸(Jasmonic acid,JA)对菠菜CBF转录因子表达的影响。[方法]以菠菜(Spinacia oleraceaL.)为材料,对其进行低温(4℃)或JA处理或用钙离子载体A23187预处理菠菜幼苗,利用Northern印迹技术检测各处理时CBF的表达情况。[结果]低温和JA均可诱导菠菜中CBF基因的表达。[结论]推测低温可能首先导致细胞中JA含量升高,JA通过一定的机制诱导细胞质基质中游离钙离子浓度([Ca2+]cyt)升高,然后Ca2+可能通过CaM(Calmodulin)或CaM相关蛋白传递低温信号,从而诱导CBF的表达。更多还原

【Abstract】 [Objective] This experiment aimed to evaluate the effects of calcium and Jasmonic acid(JA) on the expression of CBF in spinach. [Methods] The seedlings of spinach were treated with low temperature (4 ℃), JA or A23187, then used for detecting the expression of CBF by northern blotting. [Results] The results showed that the CBF expression was regulated by low temperature and JA positively. [Conclusions] Low temperature may increase the JA content of the cell firstly, then JA induced the increase of cytosolic calcium concentration ([Ca2+]cyt), and the JA induced Ca2+ transmitted the low temperature signal through CaM or CaM related proteins, regulating the CBF expression.更多还原