【摘要】 [目的]研究WRKY转录因子在番茄中的功能。[方法]根据已克隆的番茄WRKY转录因子片段设计引物,以100μmol/LJA处理6h后的番茄总RNA为模板,采用RT-PCR方法。[结果]从JA诱导的番茄幼苗中获得了608bp的cDNA片段,序列分析表明,该序列含有WRKYGQK保守结构域,与Capsicum annuum WRKY-c序列相似性是79%,与Nicotiana tabacum NtWRKY-7序列相似性是74%。[结论]已经成功克隆了番茄WRKY2基因片段。更多还原

【Abstract】 [Objective]The aim was to explore the function of WRKY transcription factor in tomato.[Method]The primers were designed in this study according to the obtained WRKY fragments,and the total RNA from tomato treated with 100 μmol/L of JA for 6 h was used as the template for RT-PCR.[Result]The 608 bp fragment was obtained from tomato with RT-PCR method.Sequence analysis indicated that this sequence contained WRKYGQK conservative domain and the similarity with Capsicum annuum WRKY-c and Nicotiana tabacum NtWRKY-7 were 79% and 74%,respectively.[Conclusion]WRKY gene sequence in tomato was cloned successfully.更多还原