【摘要】 将拮抗菌2-Q-9的1个抑菌相关基因BCCodY连接至遗传转化载体并转化烟草品种K326。PCR结果表明,该基因被成功转化。反转录PCR结果表明,BCCodY基因在K326中被转录成mRNA,说明该基因能在转基因植株中表达。为了鉴定BCCodY在转基因K326中的生物学功能,采用叶腋针刺法将烟草青枯病菌接种转基因植株,在接种后7 d内从转基因植株中分离出的青枯病菌菌落数低于非转基因植株;但接种9 d后,从两类植株中分离出的青枯菌菌落数趋于一致,病情指数也基本相同。说明转BCCodY基因的烟草植株在接种青枯菌初期能抑制青枯病菌,但后期的抑制效果不明显。更多还原

【Abstract】 This paper is an attempt to ligate the BCCodY gene of Bacillus choshinensis strain 2-Q-9 to vector pCHF3,and then transforme the recombination vector pCHF3::BCCodY into tobacco variety K326,which was successfully identified by polymerase chain reaction(PCR).Reverse transcription(RT)-PCR was performed to analyze expression of BCCodY gene in transgenic plants.The results illustrated that BCCodY gene can be transcribed by CaMV 35s promoter.To characterize the biological function of BCCodY in transgenic K326 plants,transgenic plants were inoculated with Rolstonia solanacearum strain RS2-1 by injection.The results showed that the colony number of RS2-1 isolated from transgenic K326 plants was fewer than wild plants within 7 days after inoculation,but the difference of colony number of transgenic and wild plant gradually disappeared after 9 days.It could be deduced that the BCCodY gene maybe performed antagonistic ability against R.Solanacearum in transgenic K326 plants.更多还原