【摘要】 利用高效液相色谱-二极管阵列检测(HPLC-DAD)方法结合化学计量学基于交替三线性分解算法的二阶校正方法,在未知基体干扰共存下,同时测定了中药黄芩中的黄芩素和汉黄芩素的含量。当体系组分数选取为3时,测得黄芩素和汉黄芩素的平均回收率分别为104.6±4.3%和100.7±7.4%。该方法不仅能同时给出2种物质的相对色谱图和相对光谱图,还能同时测定未知干扰存在下的2种物质的浓度。更多还原

【Abstract】 This paper proposed a novel method for simultaneous determination of baicalein and wogonin in Chinese medicinal plant Scutellaria baicalensis by using high performance liquid chromatography with photodiode-array detection(HPLC-DAD) coupled with second-order calibration method based on the alternating trilinear decomposition algorithm,especially in the presence of matrix interferences.When the core consistency diagnostic suggested N was set to 3,the average recoveries of baicalein and wogonin were 104.6±4.3% and 100.7±7.4%,respectively.更多还原