【摘要】 目的探讨联合检测肿瘤M2型丙酮酸激酶(tumor M2-PK)与癌胚抗原(carcino-embryonic antigen,CEA)和粪便潜血试验(faecal occult blood testing,FOBT)对结直肠癌早期诊断的意义。方法以44例结直肠癌患者和22名健康人群为研究对象,用酶联免疫吸附剂测定(enzyme-linked immunosorbent assay,ELISA)血清和粪便中的tumor M2-PK表达。另外检测受试者血CEA水平及粪便潜血试验。结果①血清和粪便tumor M2-PK在结直肠癌的诊断中敏感性分别为59.1%、63.6%,特异性分别为86.4%、81.8%。②血清tumor M2-PK和粪便tumor M2-PK的吻合系数k为0.522,吻合度有统计学意义。③结直肠癌患者血清和粪便中tumor M2-P的水平与肿瘤的T分期均具有相关性。结论血清tumor M2-PK+CEA+FOBT和粪便tumor M2-PK+CEA+FOBT联合检测均可提高结直肠癌诊断的敏感性;血清tumor M2-PK+CEA+FOBT更适合对健康人群的普查,粪便tumor M2-PK+CEA+FOBT更适合在结直肠癌高危人群和临床有症状人群的筛查中应用。更多还原

【Abstract】 Objective To investigate the role of the combined testing of tumor M2-PK,carcino-embryonic antigen(CEA) and faecal occult blood testing(FOBT) in the early detection of colorectal cancer(CRC).Methods and in Forty-four CRC patients and 22 normal controls were studied.Tumor M2-PK in the serum and stool was determined by enzyme-linked immunosorbent assay(ELISA),CEA in the faeces was examined,FOBT was performed.Results The sensitivity and specificity of blood tumor M2-PK in CRC diagnosis was 59.1% and 86.4%,of fecal tumor M2-PK was 63.6% and 81.8% respectively.The coherence coefficient k between these two indexes was 0.522(statistically significant).There was significant correlation between T stages of the tumor and tumor M2-P levels in the serum and stool of in the CRC patients.Conclusion The unified detection of tumor M2-PK,CEA and FOBT in the blood or faeces can increase the diagnosis sensitivity of CRC.Blood tumor M2-PK combined with CEA and FOBT detection is more suitable for healthy people census,and fecal tumor M2-PK combined with CEA and FOBT detection is more suitable for the CRC screening in crowd with high-risk or clinical symptoms.更多还原