【摘要】 建立了细胞培养基样中山姜素的定量测定方法.将三维激发发射荧光分别与化学计量学中基于平行因子分析(PARAFAC)算法和自加权交替三线性分解(SWATLD)算法的二阶校正方法相结合,当选取待分析体系组分数为2时,得到细胞培养基样中山姜素的平均回收率分别为(100.3±2.2)%,(100.1±2.2)%.两种算法所得检出限各为0.182 3μg.mL-1,0.178 1μg.mL-1.椭圆置信区间(EJCR)测试证明两种算法所得结果准确可靠.更多还原

【Abstract】 A new spectrofluorimetric method has been developed for the quantitative determination of the alpinetin content in cell culture medium samples.This method combines excitation-emission matrix fluorescence with chemometric second-order calibration methods based on Parallel Factor Analysis(PARAFAC) and Self-weighted Alternating Trilinear Decomposition(SWATLD) algorithms.When the component number was chosen to be 2,the average recoveries obtained were(100.3±2.2) % for PARAFAC and(100.1±2.2) % for SWATLD,respectively.The limits of determination for alpinetin were 0.182 3 μg·mL-1 for PARAFAC and 0.178 1 μg·mL-1 for SWATLD.EJCR has demonstrated that the results are accurate.更多还原