【摘要】 为对犬干扰素进行研究,试验采用PCR法将犬IFN-α1基因从基因组DNA克隆到pGEM-T载体,并进行测序分析。结果表明:犬IFN-α1基因ORF大小为564 bp,编码187个氨基酸,前23个为信号肽;成熟蛋白理论分子质量为19 ku,等电点为6.217,有4个潜在磷酸化位点1、个N糖基化位点和4个半胱氨酸残基,大部分为亲水区;经二级、三级结构预测,该蛋白主要由5段α螺旋组成;犬IFN-α1基因与貉子、赤狐的同源性最高,为98%,在进化树中处于同一分支;在干扰素多肽链中,15L、18L、77W、96L9、9C1、24F、131L、137S、140AWE1421、45R和150R在所有分析物种中高度保守。更多还原

【Abstract】 To conduct the research to the canine interferon-α(IFN-α),IFN-α1 gene of canine was cloned to pGEM-T vector from genomic DNA using the PCR method and was sequenced.The results showed that it was consisted of 564 bp of ORF and encoded for a 187 amino acids,the first 23 amino acids were signal peptides,the mature protein had a molecular mass of 19 ku and the isoelectric point of 6.217.It had 4 potential casein kinase Ⅱ phosphorylation sites,1 N-glycosylation site and 4 cysteine residues,and most of which were the hydrophilic regions.The protein was composed mainly of five sections of α-helix according to the secondary and tertiary structure prediction.The IFN-α1 gene of canine had the maximum similarity of 98% with that of raccoon dog and red fox,grouped into the same branch in the phylogenetic tree base on IFN-α1 gene.In the polypeptide chain of IFN-α,the amino acid residues at positions 15L,18L,77W,96L,99C,124F,131L,137S,140AWE142,145R and 150R were highly conserved in all analyzed species.更多还原