【摘要】 为了建立针对鸭疫里氏杆菌的病原学检测方法,试验根据已发表的鸭疫里氏杆菌的外膜蛋白A(OmpA)的基因序列(GenBank登录号为AF104937)设计1对引物,对7株不同血清型鸭疫里氏杆菌进行扩增并建立PCR方法。结果表明:均扩增出与预计大小一致的目的片段,PCR方法敏感性可达到8.6 pg;而多杀性巴氏杆菌、沙门菌、大肠杆菌和金色葡萄球菌均未扩增出预计大小的片段,说明该PCR方法具有良好的特异性。同时用建立的PCR方法对鸭疫里氏杆菌攻毒鸭病例和临床疑似病鸭的脏器分离菌进行扩增,均可扩增出目的条带,与细菌的分离结果相一致。更多还原

【Abstract】 To establish an aetiological method of detecting Riemerella anatipestifer,a set of specific primers were designed according to the published OmpA gene sequences of Riemerella anatipestifer(GenBank No: AF104937).Seven islolated strains with different serotypes were detected by PCR using this set of primers and a Riemerella anatipestifer specific 281 base pair DNA product was amplified by these primers.As little as 8.6 pg of Riemerella anatipestifer DNA was detected by PCR.Five other avian pathogenic bacteria were detected by PCR using this set of primers and the Riemerella anatipestifer specific 281 base pair DNA product was not amplified by these primers.It indicated that the PCR method showed good specificity.The PCR method could be used to indentification and rapid diagnosis of Riemerella anatipestifer.更多还原