【摘要】 目的比较葡萄汁与单体白黎芦醇对镉染毒小鼠的抗氧化损伤作用和促排镉作用及其作用机制。方法将32只5周龄清洁级昆明小鼠随机分为4组,分别为空白对照(双蒸水)组、镉染毒(50 mg/L)组、葡萄汁(含白藜芦醇65μg/ml)+镉染毒(50 mg/L)组、白藜芦醇(65μg/ml)+镉染毒(50 mg/L)组,每组8只,雌雄各半。氯化镉溶液采用自由饮用方式进行染毒;葡萄汁和白藜芦醇溶液采用灌胃方式进行染毒,染毒容量为0.02 ml/g,每天1次,连续染毒4周。染毒结束后,测定肝、肾组织中的镉含量,血清中尿素氮(BUN)的含量及谷丙转氨酶(ALT)的活力,肝、肾组织和血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和丙二醛(MDA)的含量。结果与镉染毒组相比,葡萄汁+镉染毒组和白藜芦醇+镉染毒组小鼠肝脏、肾脏中的镉含量均下降;血清中SOD、GSH-Px活力升高,ALT活力和BUN、MDA含量下降;肝、肾组织中SOD、GSH-Px活力升高,MDA含量下降,差异有统计学意义(P<0.01)。与白藜芦醇+镉染毒组比较,葡萄汁+镉染毒组小鼠肝脏、肾脏中的镉含量下降;血清中SOD、GSH-Px活力升高,ALT活力和BUN、MDA含量下降;肝组织中SOD活力升高,MDA含量下降;肾组织中SOD、GSH-Px活力升高,MDA含量下降,差异均有统计学意义(P<0.01)。结论葡萄汁对镉中毒小鼠排镉和肝、肾损伤的抗氧化作用强均于白黎芦醇。更多还原

【Abstract】 Objective To compare the effects of grape juice and resveratrol on antioxidation and promoting cadmium(Cd) excretion in mice exposed to Cd and the mechanism.Methods Thirty-two 5 weeks old Kunming mice were randomly divided into 4 groups,namely normal group(treated with dd H2O),CdCl2(50 mg/L) group,grape juice(containing resveratrol 65 μg/ml) plus CdCl2 group,resveratrol(65 μg/ml) plus CdCl2 treated group,8 mice in each group.The mice were treated with CdCl2 through drinking water,and treated with the grape juice and resveratrol through gavage with 0.02 ml/g,once a day for 4 consecutive weeks.In the end of treatment,the Cd levels in liver and kidney,the activity of alanine aminotranferase(ALT) and the content of urea-nitrogen(BUN) in serum were measured,and the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px),the content of malony diadehyde(MDA) in serum,liver and kidney were measured respectively.Results Compared with the CdCl2 treated group,the Cd levels in liver and kidney,the serum level of ALT and BUN,the contents of MDA in serum,liver and kidney in grape juice plus CdCl2 treated group and resveratrol plus CdCl2 group decreased while the activities of SOD and GSH-Px in serum,liver and kidney increased significantly(P<0.01).Compared with resveratrol plus CdCl2 treated group,the Cd levels in liver and kidney,the serum level of ALT and BUN,the contents of MDA in serum,liver and kidney in grape juice plus CdCl2 group decreased while the activities of SOD and GSH-Px in serum,kidney and the activities of SOD in liver increased significantly(P<0.01).Conclusion Grape juice has protective effect on the damage of liver and kidney of mice induced by Cd and has stronger effect of promote Cd excretion compared with resveratrol.更多还原