【摘要】 目的研究环境雌激素双酚A(bisphenol A,BPA)对3种激素相关肿瘤细胞株———人卵巢癌3AO细胞、前列腺癌DU145细胞及子宫内膜癌HHUA细胞增殖活性的影响。方法分别用1‰无水乙醇(溶剂对照)、BPA(终浓度为10-9mol/L)和β-雌二醇(终浓度为10-7 mol/L)染毒3AO、DU145及HHUA细胞。采用四甲基偶氮唑盐(MTT)法检测细胞(细胞密度分别为1 000、3 000、5 000个/孔)的增殖活性,并采用逆转录-聚合酶链反应检测细胞增殖因子Ki67、增殖细胞核抗原(PCNA)的mRNA表达;采用电化学发光法检测糖类抗原15-3(CA15-3)在HHUA细胞培养上清液中的含量。结果与溶剂对照组比较,BPA组3AO、DU145及HHUA细胞的吸光度(A)值较高,差异均有统计学意义(P<0.05);对3AO、DU145及HHUA细胞的促增殖率分别为10.15%,14.04%及21.31%。β-雌二醇对DU145及HHUA细胞的促增殖率分别为14.91%及9.07%,而对3AO细胞未呈现促增殖活性(P>0.05)。与溶剂对照组比较,BPA染毒组3种细胞Ki67、PCNA mRNA的表达均较高,差异有统计学意义(P<0.05);β-雌二醇染毒组DU145、HHUA细胞Ki67、PCNA mRNA的表达均较高,差异有统计学意义(P<0.05),而3AO细胞Ki67、PCNA mRNA的表达与溶剂对照组比较,差异无统计学意义。与溶剂对照组比较,BPA染毒组和雌激素对照组HHUA细胞CA15-3分泌量均较高,差异有统计学意义(P<0.05)。结论 BPA可以促进3AO、DU145及HHUA细胞的增殖活性。更多还原

【Abstract】 Objective To study the effect of bisphenol A(BPA) on the proliferation of 3AO,HHUA and DU145 cells in culture.Methods BPA(10-9 mol/L) or β-estradiol(β-E2,10-7 mol/L) was added to the culture of the three cell lines,and ethanol(1‰,V/V) was used as control group.3AO(1 000 cells/well),HHUA(3 000 cells/well) and DU145(5 000 cells/well) were seeded on 96-well plates.Cell proliferation activity was examined by MTT colorimetric assays and the expression of Ki67 and PCNA were analyzed by semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR).The Elecsys System was used for immunoassay of CA15-3 in supernatant of HHUA cells culture.Results Compared with control group,the proliferation rates of 3AO,HHUA and DU145 cells in BPA group were 10.15%,14.04% and 21.31% respectively,the effects of HHUA and DU145 cells with β-estradiol were 14.91%,9.07%,but β-estradiol had no effects on 3AO cell.Furthermore,the expression levels of Ki67 mRNA and PCNA mRNA of three cell lines in BPA group and HHUA and DU145 cells treated with β-estradiol were up-regulated significantly(P<0.05);The content of CA15-3 in HHUA cells of BPA group was obviously higher compared with control group(P<0.05).Conclusion The findings in the present paper indicate that BPA can promote the proliferation of hormone-related tumor cells.更多还原