【摘要】 对6种抗生素(3种四环素类和3种磺胺类)类污染物对活性污泥过氧化氢酶、脲酶、脱氢酶3种酶活的影响进行研究。结果表明,四环素类抗生素对活性污泥酶活的影响大于磺胺类抗生素,且对胞内酶脱氢酶的影响大于对另外2种胞外酶脲酶和过氧化氢酶,而磺胺类抗生素对过氧化氢酶的影响最大。6种抗生素类污染物对活性污泥酶活的2h培养时间的半数有效浓度(EC50)大于24h培养时间的EC50,抗生素的生物抑制毒性随培养时间的延长而加大。该方法获得的EC50较其他方法大,可能与悬浮固体对抗生素的强烈吸附有关,仅用酶活变化判定抗生素类污染物的生物毒性应谨慎。更多还原

【Abstract】 Effect of six tetracyclines and sulfonamides antibiotics on the activities of catalase,urease and dehydrogenase in activated sludge were investigated in this paper.The results indicated that the influence of tetracyclines on the enzyme activities of activated sludge was greater than that of sulfonamides.Compared with the two extracellular enzymes catalase and urease,the intracellular enzyme dehydrogenase was more susceptible to be inhibited by the tetracyclines,and catalase was found more sensitive to sulfonamides.The EC50 values of six antibiotics to the three enzymes for 2 h were higher than that for 24 h,which demonstrated that the longer the incubation time,the greater impact of antibiotics.But the EC50 value obtained in this paper was higher than that in reference,which seems to related to the great adsorption ability of suspended solid to antibiotics pollutants,so it should be cautious to determinate the inhibitory effect of antibiotics relying on the change of enzymes activity alone.更多还原