【摘要】 目的探讨骨髓间充质干细胞(marrow mesenchymal stem cells,MSCs)对联合培养细胞增殖及自身表达平滑肌肌动蛋白(smooth muscle alpha-actin,α-actin SM)的影响。方法建立MSCs与平滑肌细胞(smooth muscle cells,SMCs)直接共培养体系,对MSCs与SMCs直接共培养组和单纯SMCs组及MSCs组在不同时间点细胞计数。对共培养组和单纯MSCs组行4’6’-二乙酰基-2-苯基吲哚(DAPI)(5 ng/ml)标记,利用抗α-actin SM荧光抗体,检测2组MSCs胞浆内-actin SM表达。结果共培养组细胞计数较单独SMCs培养组明显减少,以第4天为著(P<0.01),与SMCs共培养5 d,MSCs在共培养组较单纯MSCs组表达α-actin SM阳性率明显降低(P<0.05)。结论在无干预因素情况下,MSCs与SMCs直接共培养明显抑制共培养体系中细胞增殖,且抑制MSCs表达α-actin SM。更多还原

【Abstract】 Objective To investigate the influence of marrow mesenchymal stem cells(MSCs) on cell proliferation and self differentiation in a co-culture system with smooth muscle cell(SMCs) and self-expression of smooth muscle alpha-actin(α-actin SM).Methods The direct co-culture system of MSCs with SMCs was established,and the cell count was performed in co-culture group,simple SMCs group and simple MSCs group at different time points.The cells in co-culture group and MSCs group were pre-labled by DAPI(5ng/ml),and the expression of α-actin SM was detected with anti-α-actin SM antibody by fluorescence immunocytochemistry for both groups.Results The cell counts in direct co-culture group were significantly lower than those in simple SMCs group,especially on the 4th day(1.69×104/ml vs 27.20×104/ml,P<0.01),after 5-day co-culture with SMCs,the positive rate of α-actin SM expression of MSCs in co-culture group was obviously decreased,as compared with that in simple MSCs group(44.1% vs 26.6%,P<0.05).Conclusion MSCs in the direct co-culture system with SMCs in vitro can significantly inhibit cell proliferation and reduce the α-actin SM expression of MSCs,if there is no intervention factor.更多还原