【摘要】 目的探讨C57BL/6J小鼠继发腭发育过程中,甲状腺转录因子-2(TTF-2)和重组人转化生长因子β3(TGFβ3)的表达及其相互作用规律。方法于妊娠12、13、14d分别断颈处死各6只孕鼠,无菌条件取出胚胎,解离腭突。应用免疫组织化学染色和Western印记方法检测TTF-2和TGFβ3在腭突中嵴上皮细胞的表达。结果在G57BL/6J小鼠胚胎的腭突形成、上抬和融合的发育过程中均有TTF-2的表达,以13d为最高,免疫组织化学和Western印记结果一致。TGFβ3在腭突形成时,腭突中嵴上皮细胞未检测到其表达。腭突上抬时,首次在腭中山嵴上皮细胞中发现TGFβ3表达,两侧腭突融合时,TGFβ3在腭中嵴上皮中的表达最明显,当腭突融合完成后,TGFβ3在腭中嵴上皮中表达减少。结论 TTF-2和TGFβ3两种细胞因子均在腭突中嵴上皮细胞中表达,表明与其分化转归相关。更多还原

【Abstract】 Objective To investigate the expression and interaction of TTF-2 and TGF-β3 in the development of C57BL/6J mouse palatal shelves.Methods The palatal shelves from the C57BL/6J mouse embryos were microdissected in aseptic condition on day 12,13,14 of gestation,each day killed 6 pregnant mice.The immunohistochemistry coloration and Western blot methods were used to detect TTF-2 and TGF-β3 expression in palatal medial edge epithelium(MEE).Results In the process of the C57BL/6J mouse palatal shelves’ growing,elevating and fusing,TTF-2 was expressed in MEE and became highest at embryonic day 13 consisting with immunohistochemistry coloration and Western blot results.TGFβ3 was not expressed in the MEE of shelves’ growing.TGFβ3 was first found in the MEE of shelves’ elevating and most obvious expressed at fusion.After fusing,TGFβ3 was reduced.Conclusion The two cytokine TTF-2 and TGF-β3 are both expressed in the MEE and correlating to cell differentiation and transformation.更多还原