【摘要】 目的探讨不同浓度的峨参石油醚提取物对体外培养的人肺癌细胞A549、H460的增殖及凋亡的抑制作用。方法不同浓度的峨参石油醚提取物作用于肺癌细胞A549和H460,48 h后MTT法观察药物对肺癌细胞A549和H460生长的抑制效应;用倒置显微镜观察细胞形态学的改变,应用DAPI染色法观察不同浓度峨参石油醚提取物作用于癌细胞24 h后对细胞凋亡的影响。结果峨参石油醚提取物对肺癌细胞A549和H460的半数有效抑制浓度IC50值分别为6.13μg/ml和0.97μg/ml;通过倒置显微镜可观察到细胞的形态学改变,细胞荧光染色出现了明显的凋亡细胞,表现为细胞生长明显受到抑制,荧光增加,有明显的细胞核断裂和皱缩。结论 峨参石油醚提取物对肺癌细胞增殖具有明显的抑制及诱导凋亡的作用。更多还原

【Abstract】 Objective To observe the inhibitory effect of petroleum ether extracts of Anthriscus sylvestris(L.)Hoffm.on the cell proliferation of lung cancer cell A549 and H460.Methods The cells of A549 and H460 were treated by different concentrations of petroleum ether extracts of Anthriscus,after 48 hours,the inhibitory effects on the cell growth of lung cancer cell A549 and H460 were detected by MTT,and the changes of cell morphology were observed under inverted microscope,24 hours after the cells were treated with the petroleum ether extracts of Anthriscus,and its effects on the apoptosis of cancer cells were detected by DAPI staining.Results The IC50 for the lung cancer cells A549 and H460 was 6.13μg/ml and 0.97μg/ml respectively,and the morphological changes were found under inverted microscope,and obvious apoptotic cells were observed after cells staining,and cell growth was inhibited obviously,fluorescence was increased,with visible nuclear fracture and collapse.Conclusion The petroleum ether extracts of Anthriscus have obvious inhibitory effect on the proliferation of lung cancer cell,which can induce the cells’ apoptosis.更多还原