【摘要】 目的研究卵巢浆液性腺癌中氧化还原酶的双色氨酸域基因(WW domain-containing oxidoreductase,WWOX)和10号染色体缺失的磷酸酶和张力蛋白同源物基因(phosphatase and tension homologdeleted on chromosome ten,PTEN)蛋白表达情况及其与预后的关系,为临床合理准确预测患者生存率,指导临床治疗提供依据。方法应用免疫组织化学方法检测卵巢浆液性腺癌患者56例组织中WWOX和PTEN蛋白表达情况,对其临床病理资料进行回顾性分析,运用Kaplan-Meier单因素生存分析探讨WWOX和PTEN蛋白表达与预后的关系。结果 56例卵巢浆液性腺癌中WWOX蛋白呈阳性表达27例(48.2%),明显低于正常卵巢组织中的阳性表达率(P<0.05);PTEN蛋白表达阳性29例(51.8%),明显低于正常卵巢组织中的阳性表达率(P<0.05)。WWOX蛋白表达阳性的患者3、5年生存率分别为63.0%和44.4%,明显高于阴性表达的患者(P<0.05)。PTEN蛋白表达阳性的患者3、5年生存率分别为62.1%和44.8%,均明显高于阴性表达的患者(P<0.05)。WWOX和PTEN蛋白表达在卵巢浆液性腺癌不同病理分级中的差异有统计学意义(P<0.05)。结论抑癌基因WWOX和PTEN蛋白表达缺失参与了卵巢浆液性腺癌的发生,其表达缺失的患者生存率低,预后不良。更多还原

【Abstract】 Objective In order to predict survival rate and guide clinic reasonable treatment for patients with ovarian serous adenocarcinoma,the expression of WW domain - containing oxidoreductase (WWOX) and phosphatase and tension homologdeleted on chromosome ten(PTEN) protein and their association with prognosis were explored.Methods A expression of WWOX and PTEN protein were detected with immunohistochemistry and the relationship of WWOX and PTEN expression and prognosis were analyzed in 56 ovarian serous adenocarcinoma cases with follow - up data by Kaplan - Meier univariate survival analysis.Results The immunohistochemical result showed that 27 of 56 cases were WWOX positive expression.The positive rate of WWOX expression was 48.2%,significantly lower than that in normal ovary(P <0.05).The survival rate in 3 and 5 years of patients with WWOX expression were 63.0%and 44.4%,significantly higher than that without WWOX expression.The immunohistochernical result showed that 29 of 56 cases were PTEN positive expression.The positive rate of PTEN expression was 51.8%,significantly lower than that in normal ovary(P <0.05).The survival rate in 3 and 5 years of patients with PTEN expression were 62.1%and 44.8%,significantly higher than that without PTEN expression.The significant difference of WWOX and PTEN expression was found between high and low grade ovarian serous adenocarcinoma.Conclusion The loss of WWOX and PTEN expression not only contribute to oncogenesis of ovarian serous adenocarcinoma,but also suggest that the patients have lower survival rate and poor prognosis.更多还原