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免疫试纸条结合RT-PCR法快速检测马铃薯Y病毒
The Rapid Detection of Potato Virus Y by Immunostrip Assay and RT-PCR
【摘要】 利用马铃薯Y病毒(PVY)免疫试纸条,对PVY进行了快速检测,结果表明,试纸在2 min内,可特异性检出PVY,而健康的叶片无反应。刮下试纸条上显示的检测带,进行RT-PCR,能扩增到与预期大小相同的DNA条带,是对快速检测试纸条检测结果的验证。
【Abstract】 Potato virus Y was rapidly detected by immunostrip assay,the results indicated that the PVY could be tested by immunostrip within 2 minutes and no-specific reaction was observerd for healthy leaves.RT-PCR amplification could produce a specific DNA band with an expected size,which was a validation of the results of immunostrip.
【基金】 湖北省农业科技创新中心资助项目(2007-620-001-03)
- 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,2011年05期
- 【分类号】S435.32
- 【被引频次】10
- 【下载频次】185