【摘要】 对从病死锦鲤(Cyprinus carpio L.)肝组织中分离的2株菌(编号:HC050630B-1,HC050630B-2)进行了形态特征、主要理化特性、对健康鲤鱼的致病作用、药物敏感性等方面的检验;同时测定了HC050630B-1株菌的16S rRNA基因序列,构建了系统发育树。结果表明,2株被检菌为弗氏柠檬酸杆菌(Citrobacter freundii),对健康鲤鱼有致病作用;所测菌株的16S rRNA基因序列长度1 458 bp,在GenBank中登录号为EF669481,该菌株16S rRNA基因序列与GenBank数据库中柠檬酸杆菌属细菌的16S rRNA基因序列同源性在99%;药敏试验结果显示,对供试的头孢噻肟等21种药物呈现高度敏感或敏感,对头孢唑啉等5种呈低度敏感,对四环素等11种耐药。更多还原

【Abstract】 Two strains(HC050630B-1,HC050630B-2) isolated from the liver of diseased(or dead) ornamental carp were examined,including their morphological characteristics,physiological and biochemical characteristics,pathogenicity to healthy carp and antibiotic sensitivity.In addition,the 16S rRNA gene of HC050630B-1 were sequenced,and molecular phylogenetic tree was constructed.The results showed that the two examined strains were identified as Citrobacter freundii,and had strong pathogenicity to healthy carp.The length of the 16S rRNA gene sequence of examined strain is 1 458 bp.Its database accession numbers in NCBI is EF669481,and the similarity of the 16S rRNA gene sequence of examined strain with the 16S rRNA gene of Citrobacter from GenBank database is 99%.The antibiotic sensitivity using 37 antimicrobial agents showed that isolates were high sensitive or sensitive to 21 agents including cefotaxime,slight sensitive to 5 agents including cefazolin,and resistant to 11 agents including tetrecycline.更多还原