【摘要】 在猪的肠道组织中克隆Reg3基因,提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化E.coliJM109,检测阳性克隆、测序并进行序列分析。结果表明:克隆的猪Reg3基因与人、绵羊、马的同源性分别为83.7%,82.3%,82.2%;克隆了猪Reg3全长基因并注册GenBank注册号为Accession.FJ531494。更多还原

【Abstract】 In order to clone and analyze the gene Reg3 in intestinal canal tissues of pig,the total RNA was extracted and mRNA sequence of Reg3 gene was amplified by RT-PCR method which used two designed primers.The PCR productswere ligated into the pMD-19T vector,and then transformed into competent cells of E.coli JM109.The sequence was analyzed to identify the recombinant plasmid.The identification analysis showed that the Reg3 nucleotide sequence shared 83.7%,82.3% and 82.2% homology with that of human,sheep and horse respectively,revealing porcine Reg3 gene had been successfully cloned in the present study.The sequence has been submitted to GenBank(Accession FJ531494).更多还原