【摘要】 以成熟种子为外植体建立了荻植株再生体系。荻成熟种子经20%"84"消毒液处理20 min,接种于MS+4%蔗糖(pH 5.5)培养基中培养5 d,萌发率为92%,褐化率为8%,污染率为0。萌发种子继代转入愈伤诱导培养基MS+1mg/l 6-BA+1mg/l 2,4-D+0.5mg/l NAA+4%蔗糖(pH 5.5),光照条件下愈伤组织诱导率为86%,黑暗条件下愈伤组织诱导率64%。荻愈伤组织在MS+1mg/L 6-BA+0.1mg/L2,4-D+0.5mg/L NAA+4%蔗糖(pH 5.5)进行不定芽诱导,不定芽发生率为91%。无根丛生芽继代转入不定根诱导培养1/2MS+0.25mg/L IBA+0.25mg/L NAA+4%蔗糖(pH 5.5),5天可见不定根形成,15天后不定根发生率为92%。研究还发现,荻种子萌发后可直接继代转入不定芽分化培养基形成健壮不定芽,不定芽诱导率为95%。更多还原

【Abstract】 The plantlet regeneration system was established using mature seeds of Triarrherca sacchariflora(Maxin.)Nakai as explants.The MS with 4% sucrose(pH 5.5) was used as the germination medium of seeds treated with 20% thimerosal("84" disinfection solution) for 20 minutes,in which 92% of the seed germination rate,8% of the mortality were obtained after 5 days culture.The germinated seeds were subcultured to the calli inducing medium MS+1mg/L 6-BA+1mg/L 2,4-D+0.5mg/L NAA+4% sucrose(pH 5.5).The calli formation rate was more than 86% under illumination conditions,and that was 64% under dark conditions.The adventitious buds differentiation rate was 91% with the adventitious buds induing medium MS+1 mg/L 6-BA+0.1 mg/L2,4-D+0.5 mg/L NAA4% sucrose(pH 5.5).The adventitous roots were induced with the adventitous roots inducing medium 1/2MS+0.25 mg/L IBA+0.25 mg/L NAA+4% sucrose(pH 5.5) 5 days late,the rooting rate was 92% after 15 days culture.The adventitous buds were also induced after the germinated seeds were subculutred directly to the adventitous buds differentiation medium.The inducing rate of adventitous buds was 95%.更多还原