【摘要】 建立了HPLC-ELSD同时检测乳制品中5种水溶性糖的分析方法.将奶粉样品用60～70℃水溶解后超声波提取,亚铁氰化钾溶液和乙酸锌溶液沉淀蛋白,C18固相萃取小柱净化,碳水化合物色谱柱分离,V(乙腈)∶V(水)=80∶20流动相洗脱,蒸发光散射检测器检测.结果表明:果糖、半乳糖、蔗糖、麦芽糖和乳糖在1～10mg/mL内呈现良好的线性关系,加标平均回收率为93.0%～108.9%,RSD为0.85%～2.7%.该方法简便快速,灵敏度高,且重复性好,可为奶粉及其他乳制品中水溶性糖的检测提供技术支持.更多还原

【Abstract】 A quantitative assay for the determination of sugar,such as fructose,galactose,sucrose,maltose and lactose was established by high performance liquid chromatography(HPLC).Sugars were extracted from samples with water by ultrasonic oscillation and isolated by solid phase extraction with cartridge after protein precipitation by using K4Fe(CN)6 and Zn(CH3COO)2.The separation was performed in the carbohydrate column(4.6 mm×250 mm),acetonitrile:water(80∶20)(V/V) as mobile phase,Evaporative Light Scattering detector was adopted.The results showed that the linear relation of fructose galactose sucrose maltose and lactose was well in the range of 1～10 mg/mL,the average recoveries in the 93.0%-108.9%,RSD in the range of 0.85%～2.7%.The method was accuracy,sensitivity and feasibility to analyze the residues of fructose,galactose,sucrose,maltose and lactose in dairy products.更多还原