【摘要】 分别采用传统国标GB/T 8538-2008方法、基于OprL基因的PCR方法和环介导等温扩增(LAMP)方法检测桶装水中的铜绿假单胞菌,比较三种方法的检测效果。结果显示,采集的46份市场水样中,用传统GB8538-2008方法检出了3份阳性样品,检出率为6.5%,检测时间至少2天;而PCR和LAMP方法都检出4份阳性样品,检出率为8.7%,并且可在24 h内报告结果。PCR和LAMP方法与传统方法相比具有快速灵敏等优点,可用于大批量水样的快速检测和结果初筛。更多还原

【Abstract】 Three methods including traditional method GB/T 8538-2008,PCR assay based on OprL gene and LAMP assay were used to detect the Pseudomonas aeruginosa in bottled water and the results were compared.It was found that 3 bottled water samples(6.5%) were positive detected by GB8538-2008 method,while 4 bottled water samples were detected positive by both PCR and LAMP methods with the positive rate of 8.7%.As for the test of time,the traditional method need at least 2 days to finish,compared to less than 24h using PCR and LAMP methods.Therefore,PCR and LAMP assays were more rapid and sensitive than traditional method,which may be used for rapid detection of large quantities of water samples.更多还原