【摘要】 建立了一种苦荞中芦丁和槲皮素含量的测定方法。以超声法进行提取,并分别以芦丁和槲皮素为标品,采用岛津ODS柱(4.6 mm×150 mm,5μm),以乙腈-水(含2%的冰醋酸)为流动相,梯度洗脱,流速:1 mL·min-1,检测波长:360 nm。芦丁进样量在0.023～1.150μg范围内(r=0.9996),槲皮素进样量在0.02～0.60μg范围内(r=0.9999)线性关系良好。平均加样回收率芦丁为98.5%,槲皮素为95.8%。该方法简便、快速、准确,可测定苦荞中芦丁和槲皮素的含量。更多还原

【Abstract】 A new method for determination of rutin and quercetin contents in buckwheat was developed in this paper.The extracts from buckwheat by Ultrasonic extraction was analyzed on a Shimadzu ODS column(4.6 mm×150 mm,5 μm)with a mobile phase of acetonitrile-water(containing 2% glacial acetic acid) for gradient elution at 360nm.And the flow rate was 1.0 mL/min.The linear ranges of rutin and quercetin concentrations were found within 0.023～1.150 μg(r=0.9996) and 0.02～0.60 μg(r=0.9999),respectively.The average recoveryies of rutin and quercetin were 98.5% and 95.8%,respectively.The method was simple,accurate and showed good reproducibility,which was suitable for the determination of rutin and quercetin contents in buckwheat.更多还原